AsiaChem | Chemistry in Japan | December 2021 Volume 2 Issue 1

68 | December 2021 www.facs.website Figure 4. (A) Development of albumin-based artificial metalloenzymes (ArMs) by incubation of metal catalysis into hydrophobic binding pocket (drug site I), and mechanism of glutathione resistance is based on the combined effects of the hydrophobic pocket and surface charge repulsion. (B) The two kinds of ArM-Au can catalyze amide bond formation and hydroamination, and the other two kinds of ArM-Ru can catalyze ring-closing metathesis and alkylation. (C) ArM-Ru-1 was found to be catalytically active even in the presence of up to 1000 x equivalents of glutathione. (D) The mechanistic basis behind the ArM ethylene probe (AEP) relies on the ethylene-triggered release of a quencher. (E) Fluorescent images of ripe kiwifruit slices to highlight the capabilities of AEP to detect endogenously induced ethylene. (F) The ArM-Au-2 successfully triggers drug synthesis to achieve cancer therapy in cell-based assays.

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